<< Back to previous page
Structural Genomics Consortium
Small Molecule Screening Workshop
May 9th/10th, 2011
Toronto, Canada
|
Structural Genomics Consortium (SGC) has established a platform to characterize large numbers of purified proteins (Vedadi et al. 2010; PMID: 20466062). This platform includes screening for ligands in a 384-well format using thermal shift assays. Differential scanning fluorimetry (DSF) and differential static light scattering (DSLS) are two thermal shift assays which have been optimized for screening in 384-well format (Vedadi et al. 2006, PMID: 17035505; Senisterra et al. 2006, PMID: 17092916; Niesen et al. 2007, PMID: 17853878). Application of DSLS in screening membrane proteins for ligand binding and buffer optimization in 384-well format has also been recently established (Senisterra et al. 2010, PMID: 20150591). It has been shown that identified ligands improve crystallization, crystal quality and increase the rate of success in protein structure determination. Coupled with high-resolution X-ray crystallography and structure-guided methods, the platform can also be used toward the development of chemical probes through screening families of proteins against a variety of chemical series and focused chemical libraries (Liu et al. 2009 and 2010, PMIDs: 19891491 and PMID: 20614940).
The SGC is offering a workshop on DSF and DSLS applications during the International Conference on Structural Genomics (ICSG) 2011 in Toronto. This one-day workshop aims to provide an opportunity for interested scientists to learn how to implement these techniques, free of charge, and enable them to screen a protein of interest against selected compounds during the workshop. This workshop will be held at the SGC labs in University of Toronto.
Please contact us at “mvedadi@uhnres.utoronto.ca” if you need more information.
Masoud Vedadi
Principal Investigator,
Molecular Biophysics
SGC Toronto
|
|
